Spectral intensity maps of the slices indicate distinct focal areas of high level of expression of EGFR in CRC tissues EGFR+, with very high specific NIR labeling, few high spots of EGFR in adjacent colon tissue EGFR-with practically no specific NIR labeling, and very low level of EGFR in CRC tissue EGFR-with extremely low specific NIR labeling. This evidence concerns the gene EGFR and colorectal carcinoma.