At cell lines level, six out of nine ovarian cancer cell lines also showed up-regulation of FRα mRNA and protein expression with SKOV-3, OVCAR-3 and SW626 showing strong expression while OVCA 420, Dov13 and TOV21G showed weak expression when compared with two normal ovarian epithelium cell lines in which no FRα mRNA and protein expression was detected (Figure 1B). This evidence concerns the gene FOLR1 and ovarian cancer.