We then measured the expression levels of miR-149 and ZBTB2 using quantitative RT-PCR in 5 GC cell lines (MKN45, GC9811, AGS, SGC7901, and MKN28) (Fig. 4C a) and 18 GC clinical samples (6 poorly, 6 moderately, and 6 well differentiated samples) (Fig. 4C b). This evidence concerns the gene ZBTB2 and gastric cancer.