Interestingly, in the absence of Mtb peptide stimulation, approximately 18–21% of Tim-3HighCD4+ T-cell subset from active TB patients were able to produce IFN-γ(Figure 2A, B), TNF-α (Supporting Information, Figure S5A, B), IL-2(Supporting Information, Figure S5C, D), and IL-22(Supporting Information, Figure S5E, F). Here, TNF is linked to tuberculosis.