To address these questions, Tim-3-expressing CD3+ T cells (Tim-3+CD3+ T cells) were isolated from PBMCs of active TB patients(n = 9) using magnetic beads techniques, as we described [34], and co-cultured with Mtb-infected autologous MΦs in presence or absence of anti-IL-1β Ab or isotype control Ab. The gene discussed is IL1B; the disease is tuberculosis.