To investigate whether the injected HLA-A*02:01-restricted GPC3144–152 peptide was loaded onto HLA class I molecules of tumor cells in a solid mass, an IFN-γ ELISPOT assay was performed in duplicate using these isolated tumor cells as target cells and HLA-A*02:01-restricted GPC3144–152 peptide-specific CTLs as effector cells. The gene discussed is HLA-A; the disease is neoplasm.