Then, the activity of each transcription regulator was predicted according to upstream regulator analysis and the outcomes were overlaid on the existing relationships with Cyp7a1. Illustrated in Fig. 4A, NR1I3 (LXRα) and SREBF1 were predicted to control Cyp7a1 gene expression after 2-d H-P/C-HF feeding, and the state of NR1I3 and SREBF1 was predicted as “active” and “inhibit” according to upstream regulator analysis, respectively. This evidence concerns the gene SREBF1 and hydrops fetalis.