To further understand the effect of miR-574-5p on regulating the tumor progression of human lung cancer cells, we predicted the targets of miR-574-5p by prediction programs including TargetScan and Miranda, and selected 10 possible target including CALCOCO1, RFX4, CD96, CHEX1, FOXI2, DGKG, ZNF589, ZDHHC14, CCDC88C, CLVS1 for real time PCR analysis. The gene discussed is RFX4; the disease is lung cancer.