CALCOCO1 and lung cancer: To further understand the effect of miR-574-5p on regulating the tumor progression of human lung cancer cells, we predicted the targets of miR-574-5p by prediction programs including TargetScan and Miranda, and selected 10 possible target including CALCOCO1, RFX4, CD96, CHEX1, FOXI2, DGKG, ZNF589, ZDHHC14, CCDC88C, CLVS1 for real time PCR analysis.