TWIST2 and neoplasm: Notably, a significant amount of nuclear Twist2-expressing cells at the tumor invasion front (IF) showed loss of E-cadherin in those IDC samples with surrounding lymph metastasis (76.47%, 13 of 17 cases), while in TC of the same cases, cells with Twist2 in the cytoplasm only retained E-cadherin staining on membrane or cytoplasmic E-cadherin staining (92.86%, 13 of 14 cases, Figure 3).