To determine where in the host cell LecE and LpdA perform their function during L. pneumophila infection, we constructed plasmids that over express these effectors in L. pneumophila as a fusion to a myc-tag at their N-terminus, and infected U937-derived human macrophages with a wild-type L. pneumophila containing these plasmids and used confocal fluorescence microscopy to visualize the two effectors during infection. This evidence concerns the gene MYC and infection.