Our results demonstrated that PCA3 knockdown, as demonstrated by the significant decrease in PCA3 levels after transfecting PC3 cells with the siPCA3 molecule, increased the proportion of cells with pyknotic nuclei (compared to PC3 transfected with siScr control), which was indicative of a larger number of cells undergoing apoptosis, further evidencing the role of PCA3 in modulating PCa cell survival, even in an androgen-independent cell-line model that expresses lower PCA3 levels compared to LNCaP cells. This evidence concerns the gene PCA3 and posterior cortical atrophy.