Aim of this study was to investigate the relationships between atherosclerosis and 7 main CD4+T-cell subsets with 50 derived T-cell subpopulations, resulting from the expression of specific markers (CD3/CD4/CD45RO/CD45RACCR7/ CCR5/CXCR3/HLA-DR) investigated by 10-parameter/8-color polychromatic flow cytometry. The gene discussed is CXCR3; the disease is atherosclerosis.