Transfecting A2058 cells with WNT3A leads to a 5.2-fold enhancement of BAR-luciferase, which was inhibited by siRNA-mediated knockdown of WLS and VPS35 (Supporting Information Fig S2F) suggesting that WLS-dependent WNT secretion regulates WNT/β-catenin signalling in melanoma. The gene discussed is VPS35; the disease is melanoma.