Further, flow cytometric analysis showed that SPARC transfection alone or in combination with radiation (IR) dosage of 8 Gy resulted in a significant increase of the sub-G0/G1 population of cells, which indicates the induction of apoptosis in the SK-N-AS and NB-1691 neuroblastoma cells (Fig. 2A). The gene discussed is SPARC; the disease is neuroblastoma.