The iNOS-IHC OD per volume (Figure 6) in the portal triads of the rats with chronic hepatitis was significantly increased at d28 (2-fold), d56 (1.5-fold), and d84 (3-fold) compared with that at d0, respectively, (P < 0.01); these were decreased at d56 and increased at d84 compared with that at d28, respectively (P < 0.01); so did increased that atd84 compared with that at d56 (P < 0.01). This evidence concerns the gene NOS2 and chronic hepatitis.