Interestingly, the antitumor activity induced by a recently discovered iNKT agonist, β-mannosyl-ceramide (β-ManCer), in mice bearing the CT26 colon carcinoma or B16F10 melanomas was mediated primarily by nitric oxide species (NOS) and tumor necrosis factor alpha (TNF-α) [58]. This evidence concerns the gene TNF and colon carcinoma.