The aim of this study was to specifically address the effect of HCMV infection on the cellular localization and expression of the CXCL12 chemokine and its receptors, CXCR4 and CXCR7, in the extravillous cytotrophoblast cell line SGHPL-4 and to determine whether HCMV affects EVT migration and invasion. Here, CXCR4 is linked to cytomegalovirus infection.