For normalization of gene expression, the following reference genes were selected for the various brain regions: 1) ACG: RPL13A, GAPDH and ACTB; 2) MC: B2M, RPL13A, GAPDH and ACTB; 3) THL: B2M, HPRT1 and GAPDH. The chosen reference genes for each brain region did not show any significant difference in expression between the control and autism groups. This evidence concerns the gene ACTB and autism.