DDX11 and melanoma: Specifically, we found that as shown in Figure 2C, panels b-d, transfection of the DDX11 siRNA caused melanoma cells to lose cell-cell contact in as little as 24 hr, and at doses of 25 nM as well as 150 nM of DDX11 siRNA, a significant number of the transfected melanoma cells exhibited a tightly held together chain-like morphology (Figure 2C, panels c and d).