DDX11 and melanoma: Using a pair of qPCR primers spanning exons 20–22 of human DDX11 (Figure 2A, panel a), we performed qPCR analysis, which as depicted for the 48 hr time point post transfection (Figure 2B), revealed that expression of DDX11 was decreased when compared with WM1158 MGP melanoma cells that had received only the siRNA delivery vehicle Lipofectamine 2000, or the pool of the control siRNAs.