Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis of HNC cells transfected with miR-7 demonstrated reduced EGFR mRNA expression (HN5 cells, Fig. 2B; FaDu cells, data not shown), a finding consistent with our previous studies with miR-7 in glioblastoma, lung, breast and prostate cancer cells [24]. Here, EGFR is linked to Familial prostate cancer.