An in vitro study of two putative markers identified by the in silico screen - Choline Acetyltransferase (ChAt) and urokinase-type Plasminogen Activator Receptor (PLAUR) - provided evidence supporting the validity of the method suggesting that in silico screening for biomarkers in AD and, by extrapolation, other disorders, is a productive approach. This evidence concerns the gene CHAT and Alzheimer disease.