In addition, a recent intravital imaging report by Giamperi et al. [80] showed activation of TGF-β signaling on migration of rat MTLn3 mammary tumor cells toward blood vessels in the primary tumor but subsequent downregulation of the same pathway for successful establishment of lung metastasis, again suggesting that each step of the metastatic cascade has different gene-expression programs. The gene discussed is TGFB1; the disease is neoplasm.