To assess the impact of DNA methylation on genes involved in the piRNA processing machinery, we performed bisulfite genomic sequencing of the promoter regions of PIWIL1/2 and TDRD1/9 in three CS samples, six SpF samples (three rsMF and three scMF) and three SCO samples (Fig. 2 and Fig. S1 and S2). This evidence concerns the gene PIWIL1 and Cowden syndrome 1.