Considerable studies focusing on ESR1 c.454−397 T/C (rs2234693) and c.454−351 A/G (rs9340799) polymorphisms in intron 1 are most widely discussed, and the studies found that these two SNPs are associated with ischemic stroke [26], [27], cardiovascular disease [28], [29], and atherosclerosis [30]. The gene discussed is ESR1; the disease is ischemic stroke.