Based on our previous analysis of EpCAM expression in epithelium- and mesenchyme-like breast carcinoma cell lines we selected three EpCAMhigh cell lines for lentiviral knockdown studies, i.e. T47D with a strongly glycosylated, MCF-7 with basic and glycosylated, SkBR-3 with only basic EpCAM isoform (Figure1A). Here, EPCAM is linked to breast carcinoma.