To determine if the induction of NETs formation was due to the augmented levels of cytokines, SIRS plasma (20%) was pre-incubated for 30 minute at 37°C with TNFα, IL-1β or IL-8 antibody, which led to significant reduction of NETs formation 11.4±1.5, 10.8±2.1, 12.2±1.8% respectively as compared to the non depleted plasma (Fig. 2A). This evidence concerns the gene CXCL8 and systemic inflammatory response syndrome.