In order to verify the stem-like properties of U87MG-NS we performed Real-time PCR to examine the expression of nestin and GFAP. Changes in the expression of nestin, a marker of neural stem cells, and GFAP, a marker of differentiated astrocytes, are indicative of differentiation in vitro of stem-like cell derived from glioblastoma [1]. This evidence concerns the gene NES and glioblastoma.