PITX2, ZEB1, ZNF469, and AVGR8 likely do have an effect on FECD pathogenesis, but were observed to have a much larger estimated effect on CCT, in terms of proportion of variance explained (Tables S2 and S3), which persisted after adjustment for FECD severity grade. This evidence concerns the gene ZNF469 and Fuchs endothelial corneal dystrophy.