To demonstrate that CD8+ T cell depleted mice are no longer protected by the pcDNA3-CRT/LT vaccine, C57BL/6 mice were vaccinated with pcDNA3-CRT/LT, and an in vivo antibody depletion experiment was performed as indicated in Figure6A. As shown in Figure6B, 80% of the mice depleted of CD8+ T cells did not survive up to 45 days after tumor challenge compared to 20% of non-depleted mice. Here, CD8A is linked to neoplasm.