Seven predefined apoptosis-related genes (LOX-1, CASP3, BCL-2, BAX, TIMP3, ANXA5 and cIAP-3), which were found hypermethylated in vitro[30], and INK4/ARF were screened for CpG island methylation events in a cohort of 40 CAD patients using methylation-specific PCR. This evidence concerns the gene TIMP3 and coronary artery disorder.