In a study of Raf265-resistant melanomas containing the BRAF V600E mutation, it was observed that protein kinase D3 (PRKD3) mediated resistance to both Raf and MEK inhibitors and siRNA knockdown of PRKD3 cooperated with Raf265 in suppressing the growth of the resistant melanoma cells [71]. The gene discussed is PRKD3; the disease is melanoma.