Finally, we used flow cytometric detection of the phosphorylated form of S6RP as a marker of mTOR signaling; this is a downstream mediator to mTOR, and previous AML studies using the alternative methodological approach with western blot analysis have shown that rapamycin will decrease S6RP phosphorylation [31] (Reikvam, submitted). This evidence concerns the gene MTOR and acute myeloid leukemia.