To check for the protein levels and expression patterns of Reelin proteases in the brain, we performed biochemical analyses on brain lysates of 4 weeks and 16 months old wild-type animals, as well as immunohistochemical analyses involving perfusion-fixed brains of 15 months old AD-mice (3xTg-AD) and their age-matched non-transgenic controls [33]. The gene discussed is RELN; the disease is Alzheimer disease.