CD4 and neoplasm: However, the percentages of CD4+ helper T cells (p = 0.06, one-way ANOVA) (Fig. 6B1), CD8+ cytotoxic T cells (p = 0.27, one-way ANOVA) (Fig. 6B2), or Treg cells (p = 0.22, one-way ANOVA) (Fig. 6B3) in the splenocytes were not different between among these groups after the 14-day WF-3 tumor challenge.