The kinetic low-dose CD25 Ab depletion group also had significantly higher percentages of CD4+ helper (p = 0.001, one-way ANOVA) (Fig. 6B1) and CD8+ cytotoxic T lymphocytes (p<0.001, one-way ANOVA) (Fig. 6B2) in splenocytes than in PBS and the other two sequential CD25 Ab depletion groups after 49 days of WF-3 tumor challenge. Here, CD4 is linked to neoplasm.