CEA-positive CRC cell lines (SW620, HCT116, and HT29), and three CEA-negative cancer cell lines (breast cancer Bcap37 cell line, Nasopharynageal carcinoma CNE cell line and cervical carcinoma HeLa cell line) and two normal cell lines (QSG7701 and WI38) were infected with either Ad·(ST13)·CEA·E1A(Δ24), Ad·(EGFP)·CEA·E1A(Δ24), or ONYX-015 at the indicated MOIs (0.1, 1, 5, or 10). This evidence concerns the gene CEACAM5 and cancer.