Examination of chromatin immunoprecipitation coupled to massively parallel sequencing (ChIP-seq) analysis for Nkx3.1 in mouse prostate [47] and the human prostate cancer cell line LNCaP (PDA, ML and SAA, manuscript in preparation) performed by our laboratory revealed binding sites for Nkx3.1 in both human and mouse tissue at all three genes (Figure 1B, 1C). Here, NKX3-1 is linked to prostate cancer.