BDNF and Miyoshi myopathy: First, we detected the basal levels of soluble BDNF in cultures of MM cells alone with ELISA analysis, and consistent with previous studies [19], [27], our results demonstrated the secretion of BDNF by MM cells (28.75±1.21 ng/ml for ARH-77 cells, 15.31±0.78 ng/ml for RPMI-8226 cells and 21.52±1.33 ng/ml for MMPCs).