Since no different migration ability using Boyden chamber was seen between LM8 treated with control, pazopanib, VEGF, and the combination of pazopanib and VEGF (Supplementary Fig. 6), these data indicate that VEGF-induced transendothelial migration by accelerating mAEC permeability which extending cell–cell gaps made tumor cells passing endothelium easily, and pazopanib inhibited the effect. This evidence concerns the gene VEGFA and neoplasm.