To determine whether HCMV infection inhibits Wnt signaling in SGHPL-4 cells as was observed in HFFs, SGHPL-4 cells that had been infected with HCMV for 48 hr, were stimulated with Wnt-3A ligand or LiCl for 6 hr and then immunostained for β-catenin (Figure 5). This evidence concerns the gene WNT3A and cytomegalovirus infection.