B16F10 mouse melanoma cells, which do not express pro-IL-1β, NLRP3 and AIM2 (data not shown), were chosen to generate B16F10-IL-1β, B16F10-pro-IL-1β (expressing non-secretable IL-1β) and B16F10-vector cells (Supporting Information Fig S9C and S9D). Here, AIM2 is linked to melanoma.