To evaluate the effects of IFNs on the proliferation of hepatocellular carcinoma cell lines, HepG2 and Huh7 cells were left untreated or treated with IFNα (100 ng/ml), IFNγ (100 ng/ml) and IFNλ (100 ng/ml) for 72 hrs, and viable cells were counted under microscope. The gene discussed is IFNG; the disease is hepatocellular carcinoma.