To determine if loss of PTEN altered the homeostatic translation state in glioma, we delivered RCAS-Cre virus along with RCAS-PDGFB into Ntv-a/Ink4a/Arf−/−/PTENfl/fl mice and collected ribosome-bound and total cellular RNA from Olig2+cells. Here, OLIG2 is linked to central nervous system cancer.