In 2 pancreatic cancer patients (lanes g and h) who lacked ApoAII-1 and ApoAII-2 but possessed ApoAII-4 and ApoAII-5 (Fig. 6D), immunoblot analysis under non-denaturing (native) conditions revealed loss of the 17-kDa ApoAII peptide detectable with anti-ApoAII (-ATQ) antibody (middle, Fig. 6C), and a faster-migrating (smaller) ApoAII peptide was detected with anti-Pan Apo-AII antibody (top, indicated by asterisks, Fig. 6C). The gene discussed is NLRP3; the disease is pancreatic neoplasm.