Both sphere- (Figure 3E) and adherent culture- (Figure 3F) generated xenografts highly resembled the patient tumor (Figure 3D), as indicated by hematoxylin and eosin staining, expression of the LMS diagnostic marker Smooth Muscle Actin (SMA), caldesmon, HHF35 and vimentin (Figure 3D–F and Figure S2A). Here, VIM is linked to neoplasm.