To elucidate whether the ATCC strain affected steatosis by reducing lipogenesis or increasing β-oxidation we analysed the expression of Fas, Acc1 and Cpt1a. We did not observe any regulation of expression of lipogenic enzymes, but we found a significantly higher expression of Cpt1a in the ATCC group, suggesting increased β-oxidation in this group of mice (Figure 3C–E). Here, ACACA is linked to steatosis.