We scored nuclear p-p65/RelA staining on a scale of 0 to +3 (Figure 2B, D, F), averaged the scores between double and triple punches of each sample, and defined <1.5 scores as “p-p65-low” and ≥1.5 scores as “p-p65-high.” We then evaluated the relationship between p-p65/RelA and ING4 expression in breast tumor samples and found that 47% of p-p65-high tumors expressed low levels of ING4, compared to 29% of p-p65-low tumors (p = 0.018, Figure 2G). The gene discussed is RELA; the disease is breast neoplasm.