qRT-PCR and western blotting analyses were respectively conducted to determine the levels of GOLPH3 mRNA and protein, in both normal human esophageal epithelial cells and ESCC cell lines, including KYSE-30, KYSE-140, KYSE-180, ECa-109, KYSE-510, KYSE-520, KYSE-410, 108Ca, TE-1, EC18, and HKESC-1. The gene discussed is GOLPH3; the disease is esophageal squamous cell carcinoma.