Re-feeding had no effect on the degree of c-Fos expression in the AP when compared to fasting in both 3×Tg-AD and Non-Tg mice but a significant increase in c-Fos positive cells was observed after re-feeding compared to fed controls in both mice (P<0.01 and P<0.001; Figure 4A and C). Here, FOS is linked to Alzheimer disease.