A quantitative bioassay evaluating the induction of NAD(P)H:quinone oxidoreductase 1 (Nqo1), the prototypical Nrf2 target gene, in Hepa1c1c7 murine hepatoma cells was developed and still remains a major screening tool for potential activators of the Keap1-Nrf2 pathway [18]. The gene discussed is NFE2L2; the disease is hepatocellular carcinoma.