Next we generated human hepatoma cell lines by stable transfection of HepG2 and HuH-7 cells with a human FXR expression construct and selected for stable lines overexpressing human FXR (named HepG2-FXR5 and HuH-7-37; Fig. 2A and Fig. 2D). This evidence concerns the gene NR1H4 and hepatocellular carcinoma.