To test the capacity of TIRE for measuring ligand-receptor interactions on native membranes a well-defined ligand-receptor couple was chosen, namely the binding of the chemokine CXCL12α to its natural membrane receptor CXCR4 and potentially CXCR7 [18] in Ishikawa endometrial adenocarcinoma cells. Here, CXCR4 is linked to endometrium adenocarcinoma.